Evolution of opposing regulatory interactions underlies the emergence of eukaryotic cell cycle checkpoints

Hernansaiz-Ballesteros Rosa D.; Földi Csenge Anna; Cardelli Luca; Nagy László; Csikász-Nagy Attila: Evolution of opposing regulatory interactions underlies the emergence of eukaryotic cell cycle checkpoints.
SCIENTIFIC REPORTS, 11 (1). ISSN 2045-2322 (2021)

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Folyóiratcikk

Szerző azonosítók:

Név	ORCID	MTMT szerző azonosító
Hernansaiz-Ballesteros Rosa D.
Földi Csenge Anna		10068006
Cardelli Luca
Nagy László		10034398
Csikász-Nagy Attila	0000-0002-2919-5601	10012379

Absztrakt (kivonat):

In eukaryotes the entry into mitosis is initiated by activation of cyclin-dependent kinases (CDKs), which in turn activate a large number of protein kinases to induce all mitotic processes. The general view is that kinases are active in mitosis and phosphatases turn them off in interphase. Kinases activate each other by cross- and self-phosphorylation, while phosphatases remove these phosphate groups to inactivate kinases. Crucial exceptions to this general rule are the interphase kinase Wee1 and the mitotic phosphatase Cdc25. Together they directly control CDK in an opposite way of the general rule of mitotic phosphorylation and interphase dephosphorylation. Here we investigate why this opposite system emerged and got fixed in almost all eukaryotes. Our results show that this reversed action of a kinase-phosphatase pair, Wee1 and Cdc25, on CDK is particularly suited to establish a stable G2 phase and to add checkpoints to the cell cycle. We show that all these regulators appeared together in LECA (Last Eukaryote Common Ancestor) and co-evolved in eukaryotes, suggesting that this twist in kinase-phosphatase regulation was a crucial step happening at the emergence of eukaryotes.

Folyóirat/kiadvány címe:

SCIENTIFIC REPORTS

Évszám:

2021

Kötet:

Szám:

ISSN:

2045-2322

Intézmény:

Pázmány Péter Katolikus Egyetem

Kar:

Információs Technológiai és Bionikai Kar (2013.07.-)

Nyelv:

angol

Kulcsszavak:

multidiszciplináris tudomány

MTMT rekordazonosító:

32078130

Dátum:

2024. Nov. 19. 09:12

Utolsó módosítás:

2024. Nov. 19. 09:12

URI:

https://publikacio.ppke.hu/id/eprint/1701

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