In Vitro Functional and Structural Evaluation of Low-Complexity Artificial Human Epidermis for 3D Tissue Engineering

Kocsis Dorottya; Sztankovics Dániel; Józsa Liza; Németh Afrodité; Garay Tamás; Naszlady Márton Bese; Lengyel Miléna; Vecsernyés Miklós; Antal István; Sebestyén Anna; Erdő Franciska: In Vitro Functional and Structural Evaluation of Low-Complexity Artificial Human Epidermis for 3D Tissue Engineering.
BIOENGINEERING, 12 (3). ISSN 2306-5354 (2025)

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Folyóiratcikk

Szerző azonosítók:

Név	ORCID	MTMT szerző azonosító
Kocsis Dorottya		10080212
Sztankovics Dániel		10074525
Józsa Liza	0000-0001-5835-5304	10069255
Németh Afrodité		10080217
Garay Tamás	0000-0003-0329-9207	10030126
Naszlady Márton Bese	0000-0001-8114-1750	10084103
Lengyel Miléna	0000-0001-8865-054X	10020628
Vecsernyés Miklós	0000-0001-5719-975X
Antal István	0000-0002-5434-201X	10020626
Sebestyén Anna	0000-0001-8814-4794	10000745
Erdő Franciska	0000-0001-6265-3777	10044620

Absztrakt (kivonat):

In recent times, with the need for a reduction, refinement, and replacement of in vivo animal testing, there has been an increasing demand for the use of relevant in vitro human cell systems in drug development. There is also a great demand for the replacement of skin tissue in various wounds and burns. Furthermore, human skin cell-based in vitro systems can be used to investigate the side effects (toxicity and irritation) and tissue penetration of topical preparations. In this study, exploratory experiments were performed to produce artificial epidermis using two hydrogel scaffolds, alginate and GelMA C. The amount of keratinocytes added to the matrix (10–50–100 × 106/mL) and the duration of tissue maturation (fresh, 1–3–4 weeks) were optimized in an extensive study. The behavior and structure of the two hydrogels were functionally and morphologically assessed. The permeability order for caffeine in the tested barriers was the following: alginate > GelMA C > cellulose acetate membrane > rat skin. It was concluded that GelMA C matrix provides a more favorable environment for cell survival and tissue differentiation (as demonstrated by histology and immunohistochemistry) than alginate. The 3-week incubation and 50 × 106/mL cell number proved to be the most beneficial in the given system. This study provides data for the first time on the multifactorial optimization of two potential skin substitutes for tissue manufacturing. In order to use these results in tissue engineering, the fabricated artificial epidermis preparations must also be optimized for biocompatibility and from physical and mechanical point of views.

Folyóirat címe:

BIOENGINEERING

Megjelenés éve:

2025

Kötet:

Szám:

ISSN:

2306-5354

Intézmény:

Pázmány Péter Katolikus Egyetem

Kar:

Információs Technológiai és Bionikai Kar (2013.07.-)