1H, 13C, and 15N resonance assignment of the C terminal region of the disordered postsynaptic scaffold protein GKAP

Nagy-Kanta Eszter; Sánta Anna; Kálmán Zsófia Etelka; Li Jessica Amy; Permi Perttu; Gáspári Zoltán; Péterfia Bálint: 1H, 13C, and 15N resonance assignment of the C terminal region of the disordered postsynaptic scaffold protein GKAP.
BIOMOLECULAR NMR ASSIGNMENTS, 20 (1). ISSN 1874-2718 (2025)

Szöveg
s12104-025-10253-2.pdf - NEM RÉSZLETEZETT
Download (1MB)

Mű típusa:

Folyóiratcikk

Szerző azonosítók:

Név	ORCID	MTMT szerző azonosító
Nagy-Kanta Eszter	0009-0003-1322-2008	10065218
Sánta Anna		10069730
Kálmán Zsófia Etelka		10065249
Li Jessica Amy
Permi Perttu	0000-0002-6281-1138
Gáspári Zoltán		10001271
Péterfia Bálint	0000-0001-9090-9979

Absztrakt (kivonat):

Guanylate kinase-associated protein, GKAP, is a largely disordered scaffold protein with multiple interaction partners, playing a role in the organization of the postsynaptic protein network. Its C-terminus contains a binding motif for the PDZ domain of Shank proteins, another class of postsynaptic scaffolds. Based on predictions, this motif is preceded by a ~ 40-residue disordered segment with no known additional binding sites or established functional role. Here we report the expression, purification, and 1 H, 13 C and 15 N resonance assignment of the GKAP Ct43 construct containing the C-terminal 43 residues. This region is functionally intact, having a binding affinity to Shank1 PDZ in the micromolar range. Chemical shifts indicate that this region is indeed disordered but displays helical propensity in two regions. The short, slightly helical segment immediately before a PDZ-binding motif is somewhat comparable to the structural organization observed at the C-terminus of the E6 and RSK1 proteins, but its significance in GKAP is yet to be explored.

Folyóirat címe:

BIOMOLECULAR NMR ASSIGNMENTS

Megjelenés éve:

2025

Kötet:

Szám:

ISSN:

1874-2718

Intézmény:

Pázmány Péter Katolikus Egyetem

Kar:

Információs Technológiai és Bionikai Kar (2013.07.-)