Decreased Expression of Placental Proteins in Recurrent Pregnancy Loss: Functional Relevance and Diagnostic Value

Tóth Eszter; Györffy Dániel; Posta Máté; Hupuczi Petronella; Balogh Andrea; Szalai Gábor; Orosz Gergő Balázs; Orosz László; Szilágyi András; Oravecz Orsolya; Veress Lajos; Nagy Sándor; Török Olga; Murthi Padma; Erez Offer; Papp Zoltán; Ács Nándor; Than Nándor Gábor: Decreased Expression of Placental Proteins in Recurrent Pregnancy Loss: Functional Relevance and Diagnostic Value.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25 (3). ISSN 1661-6596 (2024)

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Folyóiratcikk

Szerző azonosítók:

Név	ORCID	MTMT szerző azonosító
Tóth Eszter	0000-0001-7149-1482	10025667
Györffy Dániel		10019484
Posta Máté		10082656
Hupuczi Petronella		10012513
Balogh Andrea	0000-0003-0322-1522	10019319
Szalai Gábor		10025710
Orosz Gergő Balázs		10059724
Orosz László		10032549
Szilágyi András	0000-0002-1773-6861	10010376
Oravecz Orsolya		10081331
Veress Lajos
Nagy Sándor		10034624
Török Olga		10039073
Murthi Padma	0000-0003-2535-5134
Erez Offer
Papp Zoltán		1373182
Ács Nándor	0000-0002-1919-1869	10013998
Than Nándor Gábor		10011696

Absztrakt (kivonat):

Miscarriages affect 50–70% of all conceptions and 15–20% of clinically recognized pregnancies. Recurrent pregnancy loss (RPL, ≥2 miscarriages) affects 1–5% of recognized pregnancies. Nevertheless, our knowledge about the etiologies and pathophysiology of RPL is incomplete, and thus, reliable diagnostic/preventive tools are not yet available. Here, we aimed to define the diagnostic value of three placental proteins for RPL: human chorionic gonadotropin free beta-subunit (free-β-hCG), pregnancy-associated plasma protein-A (PAPP-A), and placental growth factor (PlGF). Blood samples were collected from women with RPL (n = 14) and controls undergoing elective termination of pregnancy (n = 30) at the time of surgery. Maternal serum protein concentrations were measured by BRAHMS KRYPTOR Analyzer. Daily multiple of median (dMoM) values were calculated for gestational age-specific normalization. To obtain classifiers, logistic regression analysis was performed, and ROC curves were calculated. There were differences in changes of maternal serum protein concentrations with advancing healthy gestation. Between 6 and 13 weeks, women with RPL had lower concentrations and dMoMs of free β-hCG, PAPP-A, and PlGF than controls. PAPP-A dMoM had the best discriminative properties (AUC = 0.880). Between 9 and 13 weeks, discriminative properties of all protein dMoMs were excellent (free β-hCG: AUC = 0.975; PAPP-A: AUC = 0.998; PlGF: AUC = 0.924). In conclusion, free-β-hCG and PAPP-A are valuable biomarkers for RPL, especially between 9 and 13 weeks. Their decreased concentrations indicate the deterioration of placental functions, while lower PlGF levels indicate problems with placental angiogenesis after 9 weeks.

Folyóirat címe:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Megjelenés éve:

2024

Kötet:

Szám:

ISSN:

1661-6596

Intézmény:

Pázmány Péter Katolikus Egyetem

Kar:

Információs Technológiai és Bionikai Kar (2013.07.-)