Decreased Expression of Placental Proteins in Recurrent Pregnancy Loss: Functional Relevance and Diagnostic Value

Tóth Eszter and Györffy Dániel and Posta Máté and Hupuczi Petronella and Balogh Andrea and Szalai Gábor and Orosz Gergő Balázs and Orosz László and Szilágyi András and Oravecz Orsolya and Veress Lajos and Nagy Sándor and Török Olga and Murthi Padma and Erez Offer and Papp Zoltán and Ács Nándor and Than Nándor Gábor: Decreased Expression of Placental Proteins in Recurrent Pregnancy Loss: Functional Relevance and Diagnostic Value.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25 (3). ISSN 1661-6596 (2024)

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Item Type:

Article

Creators:

Creators	ORCID	MTMT szerző azonosító
Tóth Eszter	0000-0001-7149-1482	10025667
Györffy Dániel		10019484
Posta Máté		10082656
Hupuczi Petronella		10012513
Balogh Andrea	0000-0003-0322-1522	10019319
Szalai Gábor		10025710
Orosz Gergő Balázs		10059724
Orosz László		10032549
Szilágyi András	0000-0002-1773-6861	10010376
Oravecz Orsolya		10081331
Veress Lajos
Nagy Sándor		10034624
Török Olga		10039073
Murthi Padma	0000-0003-2535-5134
Erez Offer
Papp Zoltán		1373182
Ács Nándor	0000-0002-1919-1869	10013998
Than Nándor Gábor		10011696

Abstract:

Miscarriages affect 50–70% of all conceptions and 15–20% of clinically recognized pregnancies. Recurrent pregnancy loss (RPL, ≥2 miscarriages) affects 1–5% of recognized pregnancies. Nevertheless, our knowledge about the etiologies and pathophysiology of RPL is incomplete, and thus, reliable diagnostic/preventive tools are not yet available. Here, we aimed to define the diagnostic value of three placental proteins for RPL: human chorionic gonadotropin free beta-subunit (free-β-hCG), pregnancy-associated plasma protein-A (PAPP-A), and placental growth factor (PlGF). Blood samples were collected from women with RPL (n = 14) and controls undergoing elective termination of pregnancy (n = 30) at the time of surgery. Maternal serum protein concentrations were measured by BRAHMS KRYPTOR Analyzer. Daily multiple of median (dMoM) values were calculated for gestational age-specific normalization. To obtain classifiers, logistic regression analysis was performed, and ROC curves were calculated. There were differences in changes of maternal serum protein concentrations with advancing healthy gestation. Between 6 and 13 weeks, women with RPL had lower concentrations and dMoMs of free β-hCG, PAPP-A, and PlGF than controls. PAPP-A dMoM had the best discriminative properties (AUC = 0.880). Between 9 and 13 weeks, discriminative properties of all protein dMoMs were excellent (free β-hCG: AUC = 0.975; PAPP-A: AUC = 0.998; PlGF: AUC = 0.924). In conclusion, free-β-hCG and PAPP-A are valuable biomarkers for RPL, especially between 9 and 13 weeks. Their decreased concentrations indicate the deterioration of placental functions, while lower PlGF levels indicate problems with placental angiogenesis after 9 weeks.

Journal or Publication Title:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Date:

2024

Volume:

Number:

ISSN:

1661-6596

Institution:

Pázmány Péter Katolikus Egyetem

Kar:

Információs Technológiai és Bionikai Kar (2013.07.-)