Integrative analysis of yeast colony growth

Gaizer Tünde Éva; Juhász János; Pillér Bíborka; Szakadáti Helga; Pongor Csaba István; Csikász-Nagy Attila: Integrative analysis of yeast colony growth.
COMMUNICATIONS BIOLOGY, 7 (1). ISSN 2399-3642 (2024)

[thumbnail of communicationsbiology2024.pdf]

Szöveg
communicationsbiology2024.pdf - NEM RÉSZLETEZETT
Download (6MB)

Mű típusa:

Folyóiratcikk

Szerző azonosítók:

Név	ORCID	MTMT szerző azonosító
Gaizer Tünde Éva		10062899
Juhász János		10049994
Pillér Bíborka		10066501
Szakadáti Helga
Pongor Csaba István	0000-0002-6820-1285	10036100
Csikász-Nagy Attila	0000-0002-2919-5601	10012379

Absztrakt (kivonat):

Yeast colonies are routinely grown on agar plates in everyday experimental settings to understand basic molecular processes, produce novel drugs, improve health, and so on. Standardized conditions ensure these colonies grow in a reproducible fashion, while in nature microbes are under a constantly changing environment. Here we combine the power of computational simulations and laboratory experiments to investigate the impact of non-standard environmental factors on colony growth. We present the developement and parameterization of a quantitative agent-based model for yeast colony growth to reproduce measurements on colony size and cell number in a colony at non-standard environmental conditions. Specifically, we establish experimental conditions that mimic the effects of humidity changes and nutrient gradients. Our results show how colony growth is affected by moisture changes, nutrient availability, and initial colony inoculation conditions. We show that initial colony spread, not initial cell number have higher impact on the final size and cell number of colonies. Parameters of the model were identified by fitting these experiments and the fitted model gives guidance to establish conditions which enable unlimited growth of yeast colonies.

Folyóirat/kiadvány címe:

COMMUNICATIONS BIOLOGY

Évszám:

2024

Kötet:

Szám:

ISSN:

2399-3642

Intézmény:

Pázmány Péter Katolikus Egyetem

Kar:

Információs Technológiai és Bionikai Kar (2013.07.-)